The refugees have been demonstrating for five days after French officials tightened border controls due to the influx of migrants to northern Europe and were made to stop their protest in front of terrified women.
The French Gendarmerie blocked the frontier on Thursday and migrants have clashed with Italian police and were staging a sit-in protest demanding to be allowed to cross the border.
Italian police forcibly removed some of the African migrants and loaded them on to buses in order to clear the Ventimiglia border crossing.
The group, mostly from Sudan and Eritrea held on to signposts and tried to resist being moved on.
It is thought that plans will see them be moved from Italy and taken to France or Germany.
It comes after the migrants, who have been pictured clinging to rocks, threatened to hurl themselves into the sea if they were refused the right to cross into northern Europe and France.
Many of them were rescued from the Mediterranean by the Royal Navy's HMS Bulwark earlier this month.
Italy is the first port of call for those seeking new lives in Europe with 57,000 migrants landing by boat already this year, out of 100,000 who have crossed the Mediterranean to the continent.
Despite EU rules requiring migrants to stay in the country where they first arrive, thousands landing on Italian shores by boat usually cross the border with France heading for northern Europe each month.
Accordind to reports, Italian government sources are viewing the closing of the French border as a hostile and 'serious action'.
Italy's interior minister said it represents a 'punch in the face for Europe'.
It comes after the Italian president of the northern Italian region of Lombardy has said he will no longer allow any migrants to enter the region following the huge rise in people trafficking to Italy.
In comments supported by other senior leaders in the region, Roberto Maroni, former leader of the right-wing Lega Nord, said he had told mayors and other officials in Lombardy to refuse the central government's demand for the settling of migrants in the region.
Last week it was revealed some how migrants rescued by HMS Bulwark in the Mediterranean and taken to Sicily strolled out of a refugee camp within 24 hours.
